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KMID : 1098420170250010029
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Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 1 p.29 ~ p.36
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Evaluation of Anti-Oxidant and Anti-Inflammatory Activities of Ganoderma lucidum Cultured on Hulled Barley
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Seo Kyoung-Hee
Kim Yeon-Hwa
Lee Young-Min
Ghosh Mithun
Park Kang-Min
Park Dong-Hyun
Kim Jin-Seong
Lim Beong-Ou
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Abstract
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Background: Ganoderma lucidum cultured on hulled barley was investigated as a potential natural source of antioxidants and anti-inflammatory agents.
Methods and Results: The yields from Ganoderma lucidum cultured on hulled barley water and ethanol extract were 17.69% and 25.77%, respectively. The antioxidant activity of Ganoderma lucidum cultured on hulled barley extracts was confirmed by various methods including assayss of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS), nitrite radical scavenging, and Fe3+ to Fe2+ reducing power activity. The ethanol extract of Ganoderma lucidum cultured on hulled barley showed improved DPPH, ABTS and nitrite radical scavenging activity compared with the water extract. After treatment of RAW264.7 cells with Ganoderma lucidum cultured on hulled barley ethanol extracts, the cell viability compared with the control was 92.82%, even at a concentration of 3,000 ㎍/㎖. The ethanol extract inhibited reactive oxygen species (ROS) generation in RAW264.7 cells stimulated with H2O2, even at low concentrations. In addition, the ethanol extract showed an inhibitory effects on the production of lipopolysaccharide-induced nitric oxide (NO) in RAW264.7 cells.
Conclusions: This study suggests that the extract of Ganoderma lucidum cultured on hulled barley is a potential source of natural antioxidants and anti-inflammatory agents.
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KEYWORD
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Ganoderma lucidum, Anti-Inflammatory, Anti-Oxidant, Hulled Barley, Lipopolysaccharide
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